The bump-and-hole method is a tool in chemical genetics for studying a specific isoform in a protein family without perturbing the other members of the family. The unattainability of isoform-selective inhibition due to structural homology in protein families is a major challenge of chemical genetics. With … See more Inspiration for the bump-and-hole method was drawn from mutant E. coli strains which carried an A294S mutant version of phenylalanine tRNA synthetase and survived exposure to p-FluoroPhe, a slightly bumped … See more As structural information about protein-ligand interfaces have become available, bump-and-hole pairs have been used to elucidate the substrates of specific proteins from … See more WebBurr holes are used to help relieve pressure on the brain when fluid, such as blood, builds up and starts to compress brain tissue. A layer of thin tissues called meninges surround …
A ‘bump-&-hole’ approach for engineering allele-selective …
WebOct 31, 2014 · In a “bump-and-hole” strategy conceptually related to that developed to selectively target the adenosine 5′-triphosphate–binding site of protein kinases (12, … WebApr 12, 2024 · His wedge approach landed within 3 feet of the hole but spun back to 11 feet, and the par putt was struck well but burned the edge on the right side. ... Woods played a low bump-and-run on the ... roehl transport discounts
Chemical biology. A bump-and-hole approach to engineer …
WebApr 1, 2024 · The bump-hole approach is a powerful chemical biology strategy to specifically probe the functions of closely related proteins. However, for many protein families, such as the AAA (ATPases ... WebOct 18, 2024 · One such effort, dubbed the bump-and-hole approach, has convincingly demonstrated that engineering at the protein-small molecule interface constitutes a powerful method for elucidating the function of a specific gene product. By manipulating the steric component of protein-ligand interactions in a complementary manner, an orthogonal … WebAlthough a previously developed bump-hole approach has proven powerful in generating specific inhibitors for mapping functions of protein kinases, its application is limited by the intolerance of the large-to-small mutation by certain kinases and the inability to control two kinases separately in the same cells. Herein, we describe the development of an … roehl transport employee reviews