WebMar 25, 2024 · This PEG-induced cell fusion provides a model system for understanding the mechanisms involved in osteoclastogenesis without using osteoclastogenic factors and for assaying new therapeutic strategies. Materials and methods WebApr 1, 2024 · Artificially induced in vitro cell fusion is one essential technique that has been extensively used for biological studies. Nevertheless, there is a lack of robust and efficient method to produce fused cells efficiently. Herein, we proposed to use cell-membrane-anchored polyvalent DNA ligands (PDL) to bring cells into close proximity by forming ...
Polyethylene glycol-mediated cell fusion
WebJun 22, 2024 · We found that PEG-6000–induced cell fusion correlated with increased type I IFN gene expression in both HepG2 liver epithelial cells ( Fig. 2 F and G and SI Appendix, Table S1) and primary human mesenchymal stem cells (hMSCs) ( SI Appendix, Fig. S1 F and G and Table S1 ). WebJan 1, 1993 · It is applicable to soluble as well as to cellular antigens and allows comparison with polyethylene glycol (PEG)-induced fusions starting from the same parental cell suspensions. The accumulated data confirm that electrofusion results in higher hybridoma yield as compared to PEG-induced fusion. fisher snow plow control wiring diagram
Engineering hybrid exosomes by membrane fusion with liposomes …
WebPolyethylene glycol (PEG) and electrofusion were applied together in a simple and highly efficient cell fusion method. PEG (8000 M(r)) was used to bring human erythrocytes into contact, and a single 4.4 kV/cm, 80 microseconds duration pulse was applied to cell suspensions. The fusion yield (FY) is P … WebThis phenomenon of specific chromosome loss, coupled with the subsequent discovery that cell fusion could be induced by inactivated Sendai virus, or by incubating cells in polyethylene glycol (PEG), provided a powerful new method for human gene mapping, which was widely used during the 1970s. WebWe concluded that the key steps in PEG-induced fusion of cell membrane are the creation of IMP-free zones, and the osmotic swelling of cells after the formation of bilayer contacts during the PEG treatment. Cell cytoskeleton affects PEG-induced fusion only to the extent of affecting IMP patching. Download to read the full article text References fisher snow plow cutting edge