Phospho- ser/thr pka substrate antibody
WebA pan-SIK–specific kinase inhibitor reduces both the hyperphosphostate and the elevated sleep need in Sleepy mutant mice, as well as sleep-deprived WT mice. SIK3 has a serine-threonine kinase domain at the N terminus and a protein kinase A (PKA) recognition site S551 in the middle portion ( 7 ). WebPhospho-PKA alpha (Ser338) Polyclonal Antibody detects endogenous levels of PKA alpha only when phosphorylated at Ser338. Target Information Protein kinase A (PKA, cAMP …
Phospho- ser/thr pka substrate antibody
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Webdetermining its substrate specificity, we assayed human recombi-nant AurA on a variety of phosphoacceptor peptide substrates in-cluding a series of properly modified derivatives of the Kemptide (ALRRASLGAA). The data presented here show that AurA is a basophilic Ser/Thr protein kinase recognizing the consensus WebPhospho-PKA substrate antibody AD0182 (supplied by Cell Signaling Technology) Phospho-PKA substrate antibody is an affinity-purified rabbit polyclonal antibody. It is highly specific for phosphorylated threonine with arginine at the –3 position. It also recognizes phospho-serine with arginine at the –2 and –3 position.
WebNov 2, 2024 · Proteins retained on sepharose were incubated with an active recombinant PKA Cα subunit for the kinase assay and then blotted with an anti-phospho- (Ser/Thr) PKA substrate antibody or anti-myc... WebPhospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also …
WebThe inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. WebDec 15, 2009 · (C) Thr-90-phosphorylation status of Hsp90α in the CM and TCL. pT90-Hsp90α was detected using anti-phospho-(Ser/Thr) PKA substrate antibody. (D) Secretion and Thr-90-phosphorylation status of Hsp90α upon overexpression of Flag-tagged PKA (Left) or upon siRNA treatment (Right) detected by Western blotting and ELISA. PKA was …
WebMar 27, 2024 · In healthy myocardium, PLB is phosphorylated at Ser 16 by PKA and at Thr 17 by CaMKII. 38, 39 Phosphorylation of TnI by PKA and PKC occurs at both Ser 23 and Ser 24 and leads to the depression of myofilament Ca 2+ sensitivity. 40-46 We tested whether these phosphorylation events were altered in the diabetic myocardium.
WebInactivation of a Gαs–PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesis flag on sleeve protocolWebFor antiphospho-PKA substrate (RRXS/T) (100G7E) antibody, a rabbit monoclonal antibody against pPKA substrate was used (commercially available from Cell Signaling). For … flag on shoulder uniformWebMonoclonal Antibody for studying PKA Substrate motif [RXX(t/s)] phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-(Ser/Thr) PKA Substrate Antibody (CST #5045) is ready to ship. flag on sleeve backwardsWebIn order to study the phosphorylated proteins in the resting Xenopus laevis oocytes, the proteins detected by Western blotting using phospho-(Ser/Thr) PKA substrate antibody … canon drucker ts5050 patronenWebImmunodetection of phospho- (Ser/Thr) PKA substrates shows that phosphorylated proteins are localized differently in spermatozoa of carp and sterlet. Strong phosphorylation of PKC substrate was observed in flagella of sterlet spermatozoa, whereas in carp sperm, PKC substrates were lightly phosphorylated in the midpiece and flagella. flag on shirtWebPolyclonal Antibody for studying PKA (RRXt) substrate motif phosphate. Cited in 210 publications. Validated for WB, IP, IHC, E. Available in 2 sizes. Highly specific and … flag on shoulder t shirtWebBuy pka antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens. ... PKA Phosphopeptide Substrate Antibody (15B12) sc-65491: mouse IgG 1: N/A: ELISA: NSR: NEW (1) ... Phospho-Specific PKA Antibodies PRODUCT NAME CATALOG # ISOTYPE EPITOPE APPLICATIONS Species Citations canon drucker ts5355a installieren