WebJan 1, 2010 · Three phenol-chloroform based protocols (TRIzol, TRItidy, EZ-RNA) and two column based protocols (UltraClean™ and E.Z.N.A.®) that were commercially available … WebTRItidy G™ is based on “single step simultaneous isolation of RNA, DNA and proteins from cell. and tissue samples”’ introduced in 1993. 1: RNA selectively retains in the aqueous …

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WebTRItidy G™ Reactivos de ácidos nucleicos Nucleic Acid Purification Kits and Reagents RNA is selectively retained in the aqueous phase during acidic GuSCN/phenol extraction; DNA and proteins are isolated from the organic … WebBuy TRItidy G™ at CymitQuimica from 150.0 € topjoy industrial co. ltd

TRItidy G - VWR

Webusing TRItidy-G reagent (Applichem Inc., Germany) from cell culture supernatant (BHK-21), according to the manufacturer’s instructions. For cDNA synthesis, a mixture of 25 pmol natural killer (NK) 61 primer (59 GAC ATG TCC TCC TGC ATC TG 39), 1 mg RNA, 50 mM Tris-HCl (pH58.3), 3 mM MgCl 2, 75 mM KCl, 0.5 mM deoxynucleotide WebFrom sample 6 onward, a phenol protocol was used by mixing 150–350 µL of collection liquid with TRItidy G™ (Panreac AppliChem). The comparison between the two methods was made by referring the amount of extracted RNA to the collection liquid used. Moreover, simultaneous extractions with both methods were performed using equal volumes (250 ... WebThey were centrifuged at 12000×g for 10 min at 4 °С. TRItidy G reagent was placed in a sterile tube and the samples were in-cubated at 25–30 °С for 5 min. Chloro-form (0.2 mL) was added to the lysate and the samples were shaken for 15 s and in-cubated at 30 °С for 10 min. The lysate was centrifuged at 12000×g for 15 min at topk t30